Failed runing xinteract

CPAS Forum (Inactive)
Failed runing xinteract g158094008  2008-01-23 23:39
Status: Closed
Assigned To: g158094008
 
Hi,
I run Mascot (version 2.1)search in the CPAS in my WinXP SP2 desktop. Most of mzXML worked successfully but few of them can not work and showed the error message as below

24 一月 2008 14:50:51,875 INFO : Mascot search for 1-1.mzXML
24 一月 2008 14:50:51,890 INFO : =======================================
24 一月 2008 14:50:51,906 INFO : MzXML2Search output
24 一月 2008 14:50:51,906 INFO : =======================================
24 一月 2008 14:51:15,140 INFO : running: MzXML2Search -mgf 1-1.mzXML
24 一月 2008 14:51:15,203 INFO :
24 一月 2008 14:51:15,203 INFO : MzXML2Search - Mascot Generic Format
24 一月 2008 14:51:15,218 INFO :
24 一月 2008 14:51:15,218 INFO : Reading 1-1.mzXML
24 一月 2008 14:51:15,218 INFO : Getting the index offset
24 一月 2008 14:51:15,218 INFO : Reading the index
24 一月 2008 14:51:18,343 INFO : scan: 1 000% 2 000% 3 000% 4 000% 5 000% 6 000% 7 << DELETED >>
24 一月 2008 15:12:08,968 INFO : PeptideProphet v3.0 April 1, 2004 (TPP v3.4 SQUALL rev.0, Build 200711281212(Win32)) AKeller@ISB
24 一月 2008 15:12:08,968 INFO : read in 2274 1+, 2544 2+, 5615 3+, 0 4+, and 0 5+ spectra
24 一月 2008 15:12:08,968 INFO : Initialising statistical models ...
24 一月 2008 15:12:09,093 INFO : iteration 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 model complete after 27 iterations
24 一月 2008 15:12:09,484 INFO : command completed in 2 sec
24 一月 2008 15:12:09,484 INFO :
24 一月 2008 15:12:09,484 INFO : running: "XPressPeptideParser "1-1.pep.xml" -nK,6.02 -dF:\Chi\SILAC_mzxml"
24 一月 2008 15:12:09,515 INFO :
24 一月 2008 15:12:09,515 INFO : command "XPressPeptideParser "1-1.pep.xml" -nK,6.02 -dF:\Chi\SILAC_mzxml" exited with non-zero exit code: -1073741515
24 一月 2008 15:12:09,531 INFO : QUIT - the job is incomplete
24 一月 2008 15:12:09,531 INFO :
24 一月 2008 15:12:09,531 INFO : command "XPressPeptideParser "1-1.pep.xml" -nK,6.02 -dF:\Chi\SILAC_mzxml" failed: Unknown error
24 一月 2008 15:12:09,531 ERROR: Failed running xinteract.

Is there anyone has the same experience?
Amy
 
 
wnels2 responded:  2008-01-25 06:11
Hi Amy,
I haven't had this exact problem but I have had problems with TPP processing of Mascot data. An approach to resolve this might be to run the command "XPressPeptideParser "1-1.pep.xml" -nK,6.02 -dF:\Chi\SILAC_mzxml" manually. Execute the command from the F:\Chi\SILAC_mzxml\mascot\1-1 directory. This may give you an error message with more information. After you are able to reproduce the error manually I would go to http://groups.google.com/group/spctools-discuss?hl=en. This is the TPP support page. They are best equipped to deal with TPP problems.

-Bill
 
Matthew Bellew responded:  2008-01-28 10:58
I edited the question to put the full log into an attachment.