Hi, I can run X!Tandem with mods and Mascot using default settings but when I try and add mods to Mascot an error.
Mascot message: (Sorry, the modification '%s' is not available)
INFO : Mascot search status: error=135
I made sure that the modification I type is the exact same one that is in the mod file on the Mascot server. Any idea what I am doing wrong? I saw an older similar posting but there was no response. Any help is appreciated.
Thanks!
Kolla |
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wnels2 responded: |
2008-10-21 06:16 |
Hi Kolla,
What version of Labkey server are you using? The newest search form will have a pull down list with the mods available on the mascot server. Can you please post the mascot.xml file that was used for the search.
Thanks,
Bill |
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kolla responded: |
2008-10-21 10:36 |
Assigned To: wnels2 |
Hi Bill,
The modules are all 8.1 but the SQL server is 8.2.7. Looks like we should update to 8.2.
We were under the impression that LabKey would automatically update since we had selected ON (medium) for the "Automatically check for updates to LabKey Server, and report anonymous usage statistics to the LabKey team" selection in the Admin Console, Customize site. Is this not correct? Can you tell me how to update manually?
Kolla |
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kolla responded: |
2008-10-21 10:49 |
Mascot.xml file used for search:
<?xml version="1.0" encoding="UTF-8"?>
<bioml>
<note label="mascot, enzyme" type="input">Trypsin</note>
<note label="spectrum, parent monoisotopic mass error plus" type="input">2.0</note>
<note label="spectrum, parent monoisotopic mass error minus" type="input">1.0</note>
<note label="spectrum, parent monoisotopic mass error units" type="input">Da</note>
<note label="spectrum, fragment mass error" type="input">1.0</note>
<note label="spectrum, fragment mass error units" type="input">Da</note>
<note label="mascot, peptide_charge" type="input">2+ and 3+</note>
<note label="scoring, maximum missed cleavage sites" type="input">2</note>
<note label="mascot, variable modifications" type="input">Carbamidomethyl(C)</note>
</bioml> |
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wnels2 responded: |
2008-10-21 11:46 |
I think it's just missing a space before the first paren. It has to be *exactly* like the Mascot form.
<note label="mascot, variable modifications" type="input">Carbamidomethyl (C)</note>
You might want to wait until 8.3. Should be very soon. I usually just download and install, I haven't tried setting for an auto update. Remember to update the labkeydbmgmt.pl script on your mascot server when you upgrade to 8.3.
Bill |
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wnels2 responded: |
2008-10-21 12:03 |
Just noticed that you had an asymmetric parent mass error in your settings. These values should be the same for Mascot or Sequest searches. |
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