ELISpot Assay Properties

ELISpot Assays support import of raw data files from Zeiss, CTL, and AID instruments, storing the data in sortable/filterable data grids.

The default ELISpot assay type includes some essential properties beyond the default properties included in general assay designs. You can also add additional properties when you create a new assay design. This topic describes the properties in the default ELISpot assay design and how they are used.

Assay Properties

Assay properties are set by an administrator at the time of assay design and apply to all batches and runs uploaded using that design. The default ELISpot assay includes the general assay properties, except for Editable Runs and Import in Background. In addition, ELISpot assays use:

  • Plate Template.
    • Choose an existing template from the drop-down list.
    • Edit an existing template or create a new one via the "Configure Templates" button. For further details, see Edit Plate Templates.
  • Detection Method: Choose the method used for detection. Options:
    • colorimetric
    • fluorescent

Batch Properties

Batch properties are set once during import of a given batch of runs and apply to all runs in the batch. The default ELISpot assay does not add additional properties to the general assay type. Data importers will be prompted to enter:

Run Properties

The user is prompted to enter values for run level properties which apply to the data in a single file, or run. Run-level properties are stored on a per-well basis, but used for record-keeping and not for calculations.

Included by default:

  • Assay ID: The unique name of the run - if not provided, the filename will be used.
  • Comments
  • ProtocolName
  • Lab ID
  • Plate ID
  • Template ID
  • Experiment Date
  • Background Subtraction: Whether to subtract background values, if a background well group is defined. See Background Subtraction for more information.
  • Plate Reader (Required): Select the correct plate reader from the dropdown list. This list is populated with values from the ElispotPlateReader list.
  • Run Data (Required): Browse or Choose the file containing data for this run.

Sample Properties

For each of the sample/specimen well groups in the chosen plate template, enter the following properties in the grid:

  • Specimen ID: Enter the specimenID for each group here. These values are often barcoded on labels attached to the plates.
  • Sample Description: A sample description for each specimen group. If you click the checkbox, all groups will share the same description.

Antigen Properties

The user will be prompted to enter these properties for each of the antigen well groups in their chosen plate template. Use the Same checkbox to apply the same value to all rows.

  • Antigen ID: The integer ID of the antigen.
  • Antigen Name
  • Cells per Well


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