This topic shows how to register a new nucleotide sequence using the graphical user interface. To register using the API, or to bulk import sequences from an Excel spreadsheet, see Use the Registry API
Nucleotide sequences can include any characters from the IUPAC notation
(except for Z).
When translating a nucleotide codon triple to a protein sequence, where the codon contains one or more of the degenerate bases, the system attempts to find a single amino acid that could be mapped to by all of the possible nucleotide combinations for that codon. If a single amino acid is found, it will be used in the translated protein. If not, the codon will be translated as an 'X'.
For example, the nucleotide sequence 'AAW' is ambiguous since it could map to either 'AAA' or 'AAT' (representing Lysine and Asparagine respectively), so 'AAW' will be translated as an 'X' However, 'AAR' maps to either 'AAA" or 'AAG' which are both are translated to Lysine, so it will be translated as a 'K'.
To add a new nucleotide sequence to the registry:
From the header bar, select Registry > Nucleotide Sequences
On the Nucleotide Sequences
page, click Insert New
Nucleotide Sequence Wizard
The wizard has two tabs:
- On the Register a new Nucleotide Sequence page, in the Details panel, enter the following:
- Description: (Optional) A text description of the sequence.
- Alias: (Optional) Alternative names for the sequence. Type a name, click enter when complete. Continue to add more as needed.
- Nucleotide Sequence Parents: (Optional) Parent components. A related sequence the new sequence is derived from, for example, related as a mutation. You can select more than one parent. Start typing to narrow the pulldown menu of options.
- Sequence: (Required) The nucleotide sequence
- Annotations: (Optional) A comma separated list of annotation information:
- Name - a freeform name
- Category - region or feature
- Type - for example, Leader, Variable, Tag, etc.
- Start and End Positions are 1-based offsets within the sequence.
- Click Next.
- To register the nucleotide and register the corresponding protein, click Finish and translate protein. This option will take you to the registry wizard for a new protein, prepopulating it with the protein sequence based on the nucleotide.
- To register the nucleotide and finish, click Finish.