The enzyme linked immunosorbent assay (ELISA) is a plate-based technique that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. The layout of controls, samples, and replicates across a 96-well plate is used to evaluate the meaning of data collected from those wells.
LabKey's ELISA assay type imports and interprets raw data from BioTek Microplate readers. A calibration curve is generated, tracking absorption over concentration. The coefficient of determination is also calculated automatically.
Like other built-in plate based assays
, each ELISA assay design you create is associated with a specific 96-well plate template. The plate template clarifies what samples and controls are in which wells and is used by the assay design to associate result values in the run data with the correct well groups and specimens.
An example application of the ELISA assay is to analyze saliva samples for protein biomarkers that might indicate cancer or autoimmune disease. Samples are applied to the plate at various concentrations. The absorption rate at each concentration is read, plotted, and can be analyzed further to better understand biomarkers.