Neutralizing Antibody assays are designed to measure the effectiveness of therapeutic drugs and are often a critical part of demonstrating immune responses. They are particularly challenging to develop and validate due to the large volumes of diverse data generated.
The NAb assay in our sample data is a plate-based assay that records neutralization in TZM-bl cells as a function of a reduction in Tat-induced luciferase (Luc) reporter gene expression after a single round of infection (Montefiori, D.C., 2004
). See related resources below.
The LabKey Server tools import the results from an Excel spreadsheet and provide management and analysis dashboards for the data. Both high- and low-throughput NAb assays are supported with options for cross- or single-plate dilutions, as well as an option for multiple viruses per plate. (For details, see NAb Plate File Formats
Basic procedures for importing and working with assay data in general are covered in the Assay Tutorial
. When working with a plate-based assay, there is an additional step of adding a plate template
to the assay design
, which is covered in the tutorial here and described in more detail in Built-In Plate-Based Assays
Dilution and well data for NAb assays is stored in the database in two tables, DilutionData and WellData. Users can write queries against these new tables, as well as export data from them.