TZM-bl Neutralization (NAb) Assay Properties

Default NAB assay designs include properties beyond the default properties included in general assay designs. For any TZM-bl Neutralization (NAb) assays, the following additional properties can be set.

Assay Properties

  • Plate Template: The template that describes the way your plate reader outputs data. You can:
    • Choose an existing template from the drop-down list.
    • Edit an existing template or create a new one via the Configure Templates button.
  • Metadata Input Format: Assays that support more than one method of adding sample/virus metadata during the data import process can be configured by selecting among possible options. Not all options are available for all configurations:
    • Manual: Metadata is entered manually at the time of data import. Available for low-throughput NAb assays only.
    • File Upload (metadata only): Metadata is uploaded in a separate file from the actual run data at the time of data import.
    • Combined File Upload (metadata & run data): Upload a combined file containing both metadata and run data. Available only for some high-throughput NAb assays.
For information about file formats used, see NAb Plate File Formats.

Run Properties

  • Cutoff Percentages 1 (required), 2, and 3
  • Host Cell
  • Study Name
  • Experiment Performer
  • Experiment ID
  • Incubation Time
  • PlateNumber
  • Experiment Date
  • FileID
  • Lock Graph Y-Axis (True/False): Fixes the Y axis from -20% to 120%, useful for generating graphs that can easily be compared side-by-side. If not set, axes are set to fit the data, so may vary between graphs.
  • Curve Fit Method. Required. The assay's run report (accessed through the details link) generates all graph, IC50, IC80 and AUC information using the selected curve fit method. You can choose from the following types of curve fits:
    • Five parameter
    • Four parameter
    • Polynomial: This algorithm allows you to quantifying a sample’s neutralization behavior based on the area under a calculated neutralization curve, commonly abbreviated as “AUC”.

Sample Fields

For each run, the user will be prompted to enter a set of properties for each of the sample well groups in their chosen plate template. In addition to general assay data property fields, which include date and participant/visit resolver information, NAb assays include:

  • Sample Description. Optional.
  • Initial Dilution. Required. Sample value: 20.0. Used for calculation.
  • Dilution Factor. Required. Sample value: 3.0. Used for calculation.
  • Method. Required. Dilution or Concentration.
For more information and a tutorial on designing and using NAb Assay Tools, see Tutorial: NAb Assay.


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