Several types of assays use plate-based technologies where spots or beads of sample are arrayed across a fixed size plate and read by an instrument. Creating an assay design for a plate-based technology adds the creation of one or more plate templates to the general assay procedure outlined in Design a New Assay
When you create an assay design, you choose the assay type
up front and then can customize a specific design to match your experiment data. There are built in assay types for several plate-based instruments, which include corresponding built in default plate layouts; you can also customize the General assay type to interpret plate-based data
and associate it with well information. The assay design process differs somewhat for these two options.
For each assay, you will first define a plate template. Follow the procedure described in this topic:
You will use the plate template you define when you design your assay as described here.
Design Built-in Plate-Based Assays
When you create an assay design from one of the built-in plate-based types, you associate it with a single template. If you want to be able to use multiple templates, you must design multiple versions of the assay design.
- From the Assay List, click New Assay Design.
- Select the desired assay type (ELISA, ELIspot, or NAb) and location.
- Click Next.
- Name the assay design (required).
- The Plate Template pulldown will include all templates available in this location. Select the template that this assay design should use.
- If you have not yet defined your plate, you can click Configure Templates here and define a new one. Note that this action will exit the assay designer, discarding any information entered so far.
- Make other assay design changes as required.
- Click Save.
Using Built-in Plate-Based Assays
When data is uploaded for a plate-based assay of one of the built in types, the plate template attached to the assay design is used. The user will not see the template name or details, nor have any opportunity to change it. If a change to the plate design is needed in the future, an administrator or assay designer must edit the assay design to change which template is used.
For a walkthrough of using a plate template with a built in assay design type, try one of these tutorials: