The LabKey Flow module automates high-volume flow cytometry analysis. It is designed to manage large data sets from standardized assays spanning many instrument runs that share a common gating strategy.

To begin using LabKey Flow, an investigator first defines a gate template for an entire study using FlowJo, and uploads the FlowJo workspace to the LabKey Server. He or she then points LabKey Flow to a repository of FCS files on a network file server, and starts an analysis.

LabKey Flow computes the compensation matrix, applies gates, calculates statistics, and generates graphs. Results are stored in a relational database and displayed using secure, interactive web pages.

Researchers can then define custom queries and views to analyze large result sets. Gate templates can be modified, and new analyses can be run and compared. Results can be printed, emailed, or exported to tools such as Excel or R for further analysis. LabKey Flow enables quality control and statistical positivity analysis over data sets that are too large to manage effectively using PC-based solutions.

Topics

• Flow Cytometry Overview
• Tutorial: Import a Flow Workspace
• Tutorial: Set Flow Background
• Tutorial: Perform a LabKey Flow Analysis
• Edit Keywords
• Add Sample Descriptions
• Custom Flow Queries
• Analysis Archive Format
• Supported FlowJo Versions

Academic papers

• Development of an automated analysis system for data from flow cytometric intracellular cytokine staining assays from clinical vaccine trials - January, 2008

Additional Resources

• FlowJo