During upload of Luminex files, we perform substitutions for certain flagged values. Other types of flagged values are imported without alteration.
Substitutions During Import for *[number] and OOR
We perform substitutions when Obs. Conc. is reported as OOR or *[number], where [number] is a numeric value. *[number] indicates that the measurement was barely out of range. OOR< and OOR> indicate that measurements were far out of range.
To determine the appropriate substitution, we first determine the lowest and highest "valid standards" for this analyte using the following steps:
N.B. The Conc in Range field will be *** for values flagged with * or OOR.
In the following table, the Well Dilution Factor and the Well FI refer to the Analyte Well (the particular experiment) where the Obs. Conc. was reported as OOR or as *[number].
When Excel Obs. Conc. is... |
We report Obs. Conc. as... | Where [value] is... |
OOR < | << [value] | the Well Dilution Factor X the Obs. Conc. of the lowest valid standard |
OOR > | >> [value] | the Well Dilution Factor X the Obs. Conc of the highest valid standard. |
*[number] and Well FI is less than the lowest valid standard FI | < [value] | the Well Dilution Factor X the Obs. Conc. of the lowest valid standard. |
*[number] and Well FI is greater than the highest valid standard FI | > [value] | the Well Dilution Factor X the Obs. Conc of the highest valid standard. |
If a valid standard is not available (i.e., standard values are out of range), [value] is left blank because we do not have a reasonable guess as to the min or max value.
Flagged Values Imported Without Change
Flag | Meaning | Column |
--- | Indicates that the investigator marked the well(s) as outliers | Appears in FI, FI Bkgd and/or Obs. Conc. |
*** | Indicates a Machine malfunction | Appears in FI, FI Bkgd, Std. Dev, %CV, Obs. Conc., and/or Conc.in Range |
[blank] | No data | Appears in any column except Analyte, Type and Well, Outlier and Dilution. |
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