This tutorial works with media and media recipes. The tutorial shows you how to:
- Register a new media mixture. In the Biologics terminology, a "mixture" is a recipe, i.e., instructions for mixing together ingredients. A mixture is a virtual entity, not a physical thing that you can place in a bottle.
- Register a physical batch of the mixture. In the Biologics terminology, a "batch" is a physical thing, i.e., the physical result of following the instructions in a recipe/mixture.
Register a Mixture
Mixtures are designed to register lab recipes and formulations. Their components are ingredients and protocol descriptions.
- Go to Media > Mixtures.
- Select Create > Mixture.
- On the Details tab, enter the following:
- Mixture Name: PBS - Cold Spring
- Description: Cold Spring formulation with optional ingredients.
- Alias: cold spring
- Mixture Type: Solution
- Click Next.
- On the Ingredients tab, enter the following:
- Recipe Measure Type: Volume
- Expiration Time: 90 Days
- Ingredients: Known
- Click Bulk Upload.
- In the pop-up dialog, copy-and-paste the following recipe into the text box.
Type | Ingredient/Mixture | Amount | Unit Type |
---|
Ingredient | Sodium Chloride | 80 | g/L |
Ingredient | Potassium Chloride | 2 | g/L |
Ingredient | Sodium Phosphate Dibasic Anhydrous | 14.4 | g/L |
Ingredient | Potassium Phosphate Monobasic | 2.4 | g/L |
Ingredient | Calcium Chloride, Dihydrate, Granular | 1.33 | g/L |
Ingredient | Magnesium chloride Anhydrous | 1 | g/L |
- Click Add Ingredients to submit the recipe.
- On the Preparation tab:
- Click Add Step.
- In Step Description enter the following:
Dissolve the reagents listed above in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.
- Click Next.
- On the Confirm tab:
- Review the entries and click Finish.
Create a Batch
- Select Create > Media > Batch.
- On the Details tab, enter the following:
- Mixture Used: PBS - Cold Spring
- Batch Name: Enter "Batch for Project H", or leave this field blank and Biologics will generate a batch name for you.
- Alias: Leave blank.
- Description: Enter "Progress evaluation of Project H"
- Vendor/Part No. : Leave blank.
- Consumed: Leave unchecked.
- Click Next.
- On the Preparation tab, enter the following:
- Desired Batch Yield: 1
- Use the dropdown to select: "L"
- Actual Batch Yield: 1
- Expiration Date: Note that this has been auto-filled, based on the Mixture's expiration property.
- Materials/Ingredient Source: Known
- Amounts/Raw Materials: Known
- Click Bulk Upload.
- Copy-and-paste the following materials list into the text box, overwriting the pre-populated text. For simplicity, we have used 'unknown' raw ingredients instead of specific lot numbers already known to the registry.
Ingredient | Amount Used | Raw Material Used |
---|
Sodium Chloride | 80 | unknown |
Potassium chloride | 2 | unknown |
Sodium phosphate dibasic anhydrous | 14.4 | unknown |
Potassium Phosphate monobasic | 2.4 | unknown |
Calcium Chloride, Dihydrate, Granular | 1.33 | unknown |
Magnesium chloride anhydrous | 1 | unknown |
- Click Add Ingredients to submit the list.
- On the Confirm tab, click Finish.
- A details page for the new batch is displayed.
- A table of batches is shown, including the new batch added in this tutorial.
Tutorial Curriculum