Set Up Flow For LabKey Analysis: /Documentation/Archive/20.7

Set Up Flow For LabKey Analysis

Documentation: Version 20.7

Set Up Flow For LabKey Analysis

This topic covers creation of a "Flow" folder and import of a FlowJo workspace that you can use for our LabKey Flow Analysis tutorial.

Step 1: Create a Flow Folder
Step 2: Upload Data Files
Step 3: Import FlowJo Workspace

Step 1: Create a Flow Folder

To begin:

Log in to your server and navigate to your "Tutorials" project. Create it if necessary.

If you don't already have a server to work on where you can create projects, start here.
If you don't know how to create projects and folders, review this topic.

Create a new subfolder named "My Flow Tutorial". Choose the folder type Flow.

Step 2: Upload Data Files

Obtain the Sample Data Files

Download the zip file: Flow sample data
Extract the zip archive to your local hard drive.

Upload to LabKey

In your "My Flow Tutorial" folder, under the Flow Summary section, click Upload and Import.

On your desktop, find the folder labkey-demo (inside the unzipped labkey-flow-demo archive).
Drag and drop this folder into the LabKey file browser, then wait for the sample files to be uploaded.
When complete, you will see the files added to your project's file management system.

Step 3: Import FlowJo Workspace

The details of importing the workspace are covered in the topic: Import a Flow Workspace and Analysis. To quickly set up your environment, follow these steps:

Click the My Flow Tutorial link to return to the main folder page.
Click Import FlowJo Workspace Analysis.

Click Browse the pipeline and select labkey-demo > Workspaces > labkey-demo.xml as shown in this screencap.

Click Next. Ignore the warnings that appear.

In Step 2, Select FCS Files, scroll down and select Browse the pipeline for a directory of FCS Files.
Click labkey-demo once to open it.
Check the box for the FACSData folder.
Click Next.