During upload of Luminex files, we perform substitutions for certain flagged values. Other types of flagged values are imported without alteration.
We perform substitutions when Obs. Conc. is reported as OOR or *[number], where [number] is a numeric value. *[number] indicates that the measurement was barely out of range. OOR< and OOR> indicate that measurements were far out of range.
To determine the appropriate substitution, we first determine the lowest and highest "valid standards" for this analyte using the following steps:
N.B. The Conc in Range field will be *** for values flagged with * or OOR.
In the following table, the Well Dilution Factor and the Well FI refer to the Analyte Well (the particular experiment) where the Obs. Conc. was reported as OOR or as *[number].
|When Excel Obs. Conc. is...||We report Obs. Conc. as...||Where [value] is...|
|OOR <||<< [value]||the Well Dilution Factor X the Obs. Conc. of the lowest valid standard|
|OOR >||>> [value]||the Well Dilution Factor X the Obs. Conc of the highest valid standard.|
|*[number] and Well FI is less than the lowest valid standard FI||< [value]||the Well Dilution Factor X the Obs. Conc. of the lowest valid standard.|
|*[number] and Well FI is greater than the highest valid standard FI||> [value]||the Well Dilution Factor X the Obs. Conc of the highest valid standard.|
If a valid standard is not available (i.e., standard values are out of range), [value] is left blank because we do not have a reasonable guess as to the min or max value.
|---||Indicates that the investigator marked the well(s) as outliers||Appears in FI, FI Bkgd and/or Obs. Conc.|
|***||Indicates a Machine malfunction||Appears in FI, FI Bkgd, Std. Dev, %CV, Obs. Conc., and/or Conc.in Range|
|[blank]||No data||Appears in any column except Analyte, Type and Well, Outlier and Dilution.|