This topic provides a reference for plate layouts, assay properties, and fields used in the LabKey ELISA Assay implementation. When you design your own plate template and assay design, you can customize and add to these defaults as needed to suit your experiment.
ELISA Plate Templates
There are two base templates you can start from to best fit your ELISA experiment design.
- In your assay folder, select (Admin) > Manage Assays.
- Click Configure Plate Templates.
- Select either the default or undiluted ELISA template to use as a starting place, depending on the structure of your experiment.
- Click Create to open the editor.
- The Template Name is required and must be unique.
- Learn about customizing plate templates in this topic: Customize Plate Templates.
- Click Save & Close when finished.
ELISA Default Template
The
ELISA Default Template is suitable for an application where you have 5 specimens and a control, typically applying dilution across the wells in the plate. There are three layers, or tabs, in the plate design template: Control, Specimen, and Replicate. The defaults for well layout are as shown:
You can change the well layouts, well group names, and add well group properties before saving your new plate template for use.
ELISA Undiluted Template
The
ELISA Undiluted Template is suitable for an application where you have up to 42 specimens and a control. Each specimen is present in two wells. There are three layers, or tabs, in the plate design template: Control, Specimen, and Replicate. The defaults for well layout are as shown:
You can change the well layouts, well group names, and add well group properties before saving your new plate template for use.
ELISA Assay Properties
The default ELISA assay includes the
standard assay properties, except for
Editable Results and
Import in Background. In addition, ELISA assays use:
- Plate Template.
- Choose an existing template from the drop-down list.
- Edit an existing template or create a new one via the "Configure Templates" button.
ELISA Assay Fields
The default ELISA assay is preconfigured with batch, run, sample, and results fields. You can customize a named design to suit the specifics of your experiment, adding new fields and changing their properties. Learn more in
Field Editor.
Batch Fields
Run Fields
- RSquared: Coefficient of determination of the calibration curve
- CurveFitParams: Curve fit parameters
Sample Fields
- SpecimenID
- ParticipantID
- VisitID
- Date
Results Fields
- SpecimenLsid
- Well Location
- WellgroupLocation
- Absorption: Well group value measuring the absorption.
- Concentration: Well group value measuring the concentration.
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