Flow Cytometry

LabKey Server helps researchers automate high-volume flow cytometry analyses, integrate the results with many kinds of biomedical research data, and securely share both data and analyses. The system is designed to manage large data sets from standardized assays that span many instrument runs and share a common gating strategy. It enables quality control and statistical positivity analysis over data sets that are too large to manage effectively using PC-based solutions.

• Manage workflows and quality control in a centralized repository
• Export results to Excel or PDF
• Securely share any data subset
• Build sophisticated queries and reports
• Integrate with other experimental data and clinical data

Topics

• LabKey Flow Module
• Flow Cytometry Overview
• Tutorial: Import a Flow Workspace
• Tutorial: Set Flow Background
• Tutorial: Perform a LabKey Flow Analysis
• Edit Keywords
• Add Sample Descriptions
• Add Statistics to FCS Queries
• Flow Module Schema
• Analysis Archive Format
• FCS keyword utility

Get Started

FlowJo

An investigator first defines a gate template for an entire study using FlowJo, and uploads the FlowJo workspace to LabKey. He or she then points LabKey Flow to a repository of FCS files.

Once the data has been imported, LabKey Server starts an analysis, computes the compensation matrix, applies gates, calculates statistics, and generates graphs. Results are stored in a relational database and displayed using secure, interactive web pages.

Researchers can define custom queries and views to analyze large result sets. Gate templates can be modified, and new analyses can be run and compared.

To get started, see the introductory flow tutorial: Tutorial: Import a Flow Workspace