An analysis script tells LabKey Server how to calculate the compensation matrix, what gates to apply, statistics to calculate, and graphs to draw.

Create a New Analysis Script

• Begin on the main page of your My Flow Tutorial folder.
• In the Flow Experiment Management panel, click "Create a New Analysis Script".
• Enter the name: "labkey-demo".
• Click Create Analysis Script.

Define a Compensation Calculation

The compensation calculation tells the LabKey Flow engine how to identify the compensation controls in an experiment. It also indicates which gates to apply. A compensation control is identified as having a particular value for a specific keyword.

• Click Upload a FlowJo workspace under Define Compensation Calculation.

• Click Choose File.
• Browse to and select the labkey-flow-demo/labkey-demo/Workspaces/labkey-demo.xml file.
• Click Submit.
• Select autocomp from the drop down and the compensation calculation will be automatically populated:

• Scroll down to Choose Source of Gating.
• Select Group labkey-demo-comps from the dropdown menu.
• Click Submit.

• Review the final compensation calculation definition.

Click on the link script main page at the bottom of the page. You can see defining the compensation calculation is now marked as completed.

Flow Scripts Web Part

Click My Flow Tutorial to return to the main folder page. Scroll down to see the web part that provides easy access to the main script page.

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