Protocol Step: Marelli_protocol (LCMS2)

demo
Name Marelli_protocol (LCMS2)
Contact
Instrument
Software
Description Prepare and run LCMS, producing one mzXml file per input sample.
Max input data per instance
Max input material per instance
Output data per instance
Output material per instance
Category mass_spec
TreatAsFractions false
ApplicationLSIDTemplate ${RunLSIDBase}:MS2.PreSearch
ApplicationNameTemplate Do MS2 Run
Material Inputs
Data Inputs
Material Outputs
Data Outputs
Protocol Predecessors
Name
Description
Marelli_protocol (LCMS2)1Prepare and run LCMS, producing one mzXml file per input sample.
Protocol Successors
Name
Description
Marelli_protocol (LCMS2)1Prepare and run LCMS, producing one mzXml file per input sample.
Marelli_protocol Sample Prep10An organellar 20KgP fraction was subjected to isopycnic density gradient centrifugation and analyzed by SDS-PAGE and Coomassie blue staining. Fractions enriched for peroxisomes (EP) or mitochondria (M) were identified by Western blotting. Equal amounts of protein derived from each of the hypotonically lysed M and EP fractions were combined and analyzed by ICAT MS/MS.