Protocol Step: Marelli_protocol Sample Prep: /home/Demos/CPAS/demo

Protocol Step: Marelli_protocol Sample Prep

demo

Standard Properties

Name	Marelli_protocol Sample Prep
Contact
Instrument
Software
Description	An organellar 20KgP fraction was subjected to isopycnic density gradient centrifugation and analyzed by SDS-PAGE and Coomassie blue staining. Fractions enriched for peroxisomes (EP) or mitochondria (M) were identified by Western blotting. Equal amounts of protein derived from each of the hypotonically lysed M and EP fractions were combined and analyzed by ICAT MS/MS.
Max input data per instance	0
Max input material per instance	1
Output data per instance	0
Output material per instance	1

Protocol Parameters

ApplicationLSIDTemplate	${RunLSIDBase}:SamplePreparation.Default
OutputMaterialNameTemplate	${InputName}.Modified
ApplicationNameTemplate	SamplePreparation
OutputMaterialLSIDTemplate	${RunLSIDBase}:${InputName}.Modified

Protocol Details

Material Inputs

Name Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Sample Set Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart
No data to show.

Data Inputs

Name Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Data Class Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart
No data to show.

Material Outputs

Name Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Sample Set Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart
No data to show.

Data Outputs

Name Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Data Class Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart
No data to show.

Protocol Predecessors

Name	Predecessor Sequence Sort Ascending Sort Descending Clear Sort Filter... Clear Filter	Description
Marelli_protocol (LCMS2)	1	Prepare and run LCMS, producing one mzXml file per input sample.

Protocol Successors

Name	Action Sequence Sort Ascending Sort Descending Clear Sort Filter... Clear Filter	Description
Marelli_protocol LC MS2	30	ICAT-labeled peptides were analyzed by µLC-ESI-MS/MS. Peptides were separated by on-line reversed-phase chromatography using a 75 µm x 10 cm self-packed Magic C18AQ column (Michrom BioResources) at a flow rate of 300 nL/min. Peptide fragmentation by collision-induced dissociation was performed in an automated fashion using the dynamic-exclusion option from the full-range MS spectra or by GPFm/z using an ion trap mass spectrometer (Thermofinnigan; Yi et al., 2002).