Peptide Validation

Peptide Validation
  1. Import FCS Files
    2326 FCS files have been imported. These are in 11 runs.
Flow Controls Statistics over Time
CD4 IFNg+
CD4 IL2+
CD4 IL4+
CD4 TNFa+
CD8 IFNg+
CD8 IL2+
CD8 IL4+
CD8 TNFa+

CompControlPassFailA compensation control passes if there are at least 1000 events in the ungated population, and the gated population is at least an amount dependent on the stain.
CompensationControlsThe 'CompensationControls' table shows statistics and graphs of FCS files that were used to calculate a compensation matrix.
CompensationMatricesThe 'CompensationMatrices' table shows compensation matrices and their spill values.
demo
DeviationFromMeanEnsures that for each FCS file, the following stats do not differ by more than 10% (of the average) from the average of the files for the same sample: Live frequency, CD4 frequency, CD8 frequency
FCSAnalysesThe 'FCSAnalyses' table shows statistics and graphs of FCS files.
FCSFilesThe 'FCSFiles' table shows FCS files and their keywords.
PassFailEnsures that all FCS files for a given sample meet the following criteria: min 10000 CD4 and CD8 (5000 for Env, SEB, CMV), sebctrl >= 1.2%, negctrl <= .1%. Details link takes you to the "PassFailDetails" query.
PassFailDetailsLike the PassFail query, but has one row per FCS file.
ResponseCD4 or CD8 positive response. Makes use of background specified in metadata.
RunsThe flow 'Runs' table shows experiment runs in the three steps of analysis: read Keywords, calculate Compensation, and perform Analysis.
StatisticDemo
SubsetDemo
Summary3CytokineComplete set of data for statistical analysies. Includes one row for each boolean gate.

Summary


Analysis Scripts

Analysis Folders