Hi all,
I have checked the term of "Failed running xinteract" in this forum, but no good answer can solve my problem. I run Mascot (version 2.1)search in the CPAS in my WinXP SP2 desktop. Most of mzXML worked successfully but few of them can not work and showed the error message as below
16 一月 2008 23:25:58,221 INFO : Mascot search for 4-11.mzXML
16 一月 2008 23:25:58,221 INFO : =======================================
16 一月 2008 23:44:13,815 INFO : MzXML2Search output
16 一月 2008 23:44:13,815 INFO : =======================================
16 一月 2008 23:44:16,018 INFO : running: MzXML2Search -mgf 4-11.mzXML
16 一月 2008 23:44:17,081 INFO :
16 一月 2008 23:44:17,081 INFO : MzXML2Search - Mascot Generic Format
16 一月 2008 23:44:17,081 INFO :
16 一月 2008 23:44:17,081 INFO : Reading 4-11.mzXML
16 一月 2008 23:44:17,081 INFO : Getting the index offset
16 一月 2008 23:44:17,081 INFO : Reading the index
16 一月 2008 23:44:23,456 INFO : scan: 1 000% 2 000% 3 000% 4 000% 5 000% 6 000% 100%
16 一月 2008 23:44:23,471 INFO : Done.
16 一月 2008 23:44:23,471 INFO :
16 一月 2008 23:44:23,471 INFO : mascot client output
16 一月 2008 23:44:23,471 INFO : =======================================
16 一月 2008 23:44:24,612 INFO : ../bin/ms-searchcontrol.exe - Version 2.1.03 - Server: Microsoft-IIS/6.0
16 一月 2008 23:44:25,706 INFO : Creating Mascot session...
16 一月 2008 23:44:26,081 INFO : Mascot session#all_secdisabledsession started.
16 一月 2008 23:44:26,081 INFO : Creating Mascot search task...
16 一月 2008 23:44:26,565 INFO : Mascot search task#120049826801 created with 'nph-mascot.exe'.
16 一月 2008 23:44:26,565 INFO : Submitting search to Mascot server...
16 一月 2008 23:44:26,581 INFO : Submitting query file, size=37935511
16 一月 2008 23:45:16,925 INFO : Mascot search task status: query upload completed
16 一月 2008 23:47:22,362 INFO : Search submitted.
16 一月 2008 23:47:22,362 INFO : Mascot search status verbose reporting on
16 一月 2008 23:47:52,737 INFO : Mascot search status: complete
16 一月 2008 23:48:02,737 INFO : Retrieving Mascot search result...
16 一月 2008 23:48:30,471 INFO : Downloaded 38739440 bytes of result file.
16 一月 2008 23:48:30,471 INFO : Mascot search result retrieved.
16 一月 2008 23:48:30,846 INFO : Mascot session ended.
16 一月 2008 23:48:30,862 INFO : Sequence Database Synchronization output
16 一月 2008 23:48:30,862 INFO : =======================================
16 一月 2008 23:48:30,862 INFO : Retreiving database information (ipi.HUMAN.v3.27.fasta)...
16 一月 2008 23:48:31,018 INFO : Database ipi.HUMAN.v3.27.fasta, hash=3b33280021ad9c17ce459e163a1cb3797a51a2bb, size=38782632, timestamp=1175014178
16 一月 2008 23:48:31,018 INFO : Local copy of database ipi.HUMAN.v3.27.fasta exists, skipping download.
16 一月 2008 23:48:31,018 INFO : Mascot2XML output
16 一月 2008 23:48:31,018 INFO : =======================================
16 一月 2008 23:48:31,018 INFO : running: Mascot2XML 4-11.dat -DC:\Secretome\databases\mascot\IPI_human\ipi.HUMAN.v3.27.fasta -xml -notgz -desc
16 一月 2008 23:48:31,081 INFO : filepath: C:\CHEN\protcols\mascot\mascot_modification_O_C\4-11.work\, extn: 4-11
16 一月 2008 23:48:31,081 INFO : A stored as 71.0779
16 一月 2008 23:48:31,081 INFO : B stored as 114.595
16 一月 2008 23:48:31,081 INFO : C stored as 103.143
16 一月 2008 23:48:31,081 INFO : D stored as 115.087
16 一月 2008 23:48:31,081 INFO : E stored as 129.114
16 一月 2008 23:48:31,081 INFO : F stored as 147.174
16 一月 2008 23:48:31,081 INFO : G stored as 57.0513
16 一月 2008 23:48:31,081 INFO : H stored as 137.139
16 一月 2008 23:48:31,081 INFO : I stored as 113.158
16 一月 2008 23:48:31,081 INFO : J stored as 0
16 一月 2008 23:48:31,081 INFO : K stored as 128.172
16 一月 2008 23:48:31,081 INFO : L stored as 113.158
16 一月 2008 23:48:31,081 INFO : M stored as 131.196
16 一月 2008 23:48:31,081 INFO : N stored as 114.103
16 一月 2008 23:48:31,081 INFO : O stored as 0
16 一月 2008 23:48:31,096 INFO : P stored as 97.1152
16 一月 2008 23:48:31,096 INFO : Q stored as 128.129
16 一月 2008 23:48:31,096 INFO : R stored as 156.186
16 一月 2008 23:48:31,096 INFO : S stored as 87.0773
16 一月 2008 23:48:31,096 INFO : T stored as 101.104
16 一月 2008 23:48:31,096 INFO : U stored as 150.038
16 一月 2008 23:48:31,096 INFO : V stored as 99.1311
16 一月 2008 23:48:31,096 INFO : W stored as 186.21
16 一月 2008 23:48:31,096 INFO : X stored as 111
16 一月 2008 23:48:31,096 INFO : Y stored as 163.173
16 一月 2008 23:48:31,096 INFO : Z stored as 128.622
16 一月 2008 23:48:31,096 INFO : c stored as 17.0073
16 一月 2008 23:48:31,096 INFO : n stored as 1.00794
16 一月 2008 23:48:31,096 INFO : variable modification 1, delta mass 57.0513 for Carbamidomethyl (C):
16 一月 2008 23:48:31,096 INFO : residues: C
16 一月 2008 23:48:31,096 INFO : nterm: 0
16 一月 2008 23:48:31,096 INFO : cterm: 0
16 一月 2008 23:48:31,096 INFO : protterm: 0
16 一月 2008 23:48:31,096 INFO :
16 一月 2008 23:48:31,096 INFO : added variable modification for C
16 一月 2008 23:48:31,940 INFO : Trypsin....
16 一月 2008 23:48:31,940 INFO : recorded static mods:
16 一月 2008 23:48:31,940 INFO : aa: B mass: 114.595 index: 0 static
16 一月 2008 23:48:31,940 INFO :
16 一月 2008 23:48:31,940 INFO : recorded static mods:
16 一月 2008 23:48:31,940 INFO : aa: J mass: 0 index: 0 static
16 一月 2008 23:48:31,940 INFO :
16 一月 2008 23:48:31,940 INFO : recorded static mods:
16 一月 2008 23:48:31,940 INFO : aa: O mass: 0 index: 0 static
16 一月 2008 23:48:31,940 INFO :
16 一月 2008 23:48:31,940 INFO : recorded static mods:
16 一月 2008 23:48:31,940 INFO : aa: U mass: 150.038 index: 0 static
16 一月 2008 23:48:31,940 INFO :
16 一月 2008 23:48:31,940 INFO : recorded static mods:
16 一月 2008 23:48:31,940 INFO : aa: X mass: 111 index: 0 static
16 一月 2008 23:48:31,940 INFO :
16 一月 2008 23:48:31,940 INFO : recorded static mods:
16 一月 2008 23:48:31,940 INFO : aa: Z mass: 128.622 index: 0 static
16 一月 2008 23:48:31,940 INFO :
16 一月 2008 23:48:31,940 INFO : recorded variable mods:
16 一月 2008 23:48:31,940 INFO : aa: C mass: 160.194 index: 1 variable
16 一月 2008 23:48:31,940 INFO :
16 一月 2008 23:48:31,940 INFO : Sample enzyme has not been specified, "Trypsin" from .dat file assumed
16 一月 2008 23:48:31,940 INFO : trypsin : cut(KR) nocuts(P) sense(C)
16 一月 2008 23:48:31,940 INFO : prev_aa and next_aa have already been read from .dat file.
16 一月 2008 23:48:31,940 INFO : Completing 594 protein descriptions by parsing through the database...
16 一月 2008 23:48:31,940 INFO : Replacing 594 protein id with full ids by parsing through the database...
16 一月 2008 23:48:32,800 INFO : searching c:/Secretome/databases/mascot/IPI_human/ipi.HUMAN.v3.27.fasta.....5%.....10%.....15%.....20%.....30%.....35%.....40%.....45%.....50%...
..60%.....65%.....70%.....75%.....80%.....90%.....95%...done
16 一月 2008 23:48:32,800 INFO : Skipping .dta, .out and .tgz generation
16 一月 2008 23:48:32,893 INFO : xinteract output
16 一月 2008 23:48:32,893 INFO : =======================================
16 一月 2008 23:48:32,893 INFO : running: xinteract -Opt -nR -x20 -N4-11.pep.xml 4-11-raw.xml
16 一月 2008 23:48:32,909 INFO :
16 一月 2008 23:48:32,909 INFO : xinteract (TPP v3.4 SQUALL rev.0, Build 200711281212(Win32))
16 一月 2008 23:48:32,909 INFO :
16 一月 2008 23:48:32,909 INFO : running: "InteractParser "4-11.pep.xml" "4-11-raw.xml""
16 一月 2008 23:48:32,987 INFO : file 1: 4-11-raw.xml
16 一月 2008 23:48:33,128 INFO : processed altogether 1146 results
16 一月 2008 23:48:33,237 INFO : command completed in 1 sec
16 一月 2008 23:48:33,237 INFO :
16 一月 2008 23:48:33,237 INFO : running: "PeptideProphetParser "4-11.pep.xml" EXTRAITRS=20"
16 一月 2008 23:48:33,268 INFO : (MASCOT)
16 一月 2008 23:48:33,268 INFO : results for charge 1: 1 id tot and 0 adj scores
16 一月 2008 23:48:33,300 INFO : results for charge 2: 559 id tot and 3 adj scores
16 一月 2008 23:48:33,331 INFO : results for charge 3: 585 id tot and 1 adj scores
16 一月 2008 23:48:33,331 INFO : results for charge 4: 0 id tot and 0 adj scores
16 一月 2008 23:48:33,346 INFO : results for charge 5: 0 id tot and 0 adj scores
16 一月 2008 23:48:33,346 INFO : init with MASCOT trypsin
16 一月 2008 23:48:33,393 INFO : MS Instrument info: Manufacturer: ThermoFinnigan, Model: LTQ Orbitrap, Ionization: ESI, Analyzer: Ion Trap, Detector: EMT
16 一月 2008 23:48:33,393 INFO :
16 一月 2008 23:48:33,393 INFO : PeptideProphet v3.0 April 1, 2004 (TPP v3.4 SQUALL rev.0, Build 200711281212(Win32)) AKeller@ISB
16 一月 2008 23:48:33,393 INFO : read in 1 1+, 559 2+, 585 3+, 0 4+, and 0 5+ spectra
16 一月 2008 23:48:33,393 INFO : Initialising statistical models ...
16 一月 2008 23:48:33,409 INFO : iteration 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 model complete after 27 iterations
16 一月 2008 23:48:33,471 INFO : command completed in 0 sec
16 一月 2008 23:48:33,471 INFO :
16 一月 2008 23:48:33,471 INFO : running: "ProteinProphet "4-11.pep.xml" "4-11.pep-prot.xml" XML NOPLOT"
16 一月 2008 23:48:33,503 INFO : ProteinProphet (C++) by Insilicos LLC and LabKey Software, after the original Perl by A. Keller (TPP v3.4 SQUALL rev.0, Build 200711281304(Win32))
16 一月 2008 23:48:33,550 INFO : . . . reading in C:/CHEN/protcols/mascot/mascot_modification_O_C/4-11.work/4-11.pep.xml. . .
16 一月 2008 23:48:33,550 INFO : . . . read in 0 1+, 0 2+, 0 3+, 0 4+ spectra with min prob 0.05
16 一月 2008 23:48:33,550 INFO : (xml input) (using degen pep info)
16 一月 2008 23:48:33,565 INFO : no data - quitting
16 一月 2008 23:48:33,565 INFO : command "ProteinProphet "4-11.pep.xml" "4-11.pep-prot.xml" XML NOPLOT" exited with non-zero exit code: 1
16 一月 2008 23:48:33,565 INFO : QUIT - the job is incomplete
16 一月 2008 23:48:33,565 INFO :
16 一月 2008 23:48:33,565 INFO : command "ProteinProphet "4-11.pep.xml" "4-11.pep-prot.xml" XML NOPLOT" failed: Operation not permitted
16 一月 2008 23:48:33,565 ERROR: Failed running xinteract.
Is there anyone has the same experience?
--Jack |