How to create MRM methods and analysis? | Kynda | 2017-10-31 15:54 | |||||||||
Status: Closed | |||||||||||
Hi, I am new to proteomics. I want to perform MRM for organelle specific proteins and some pathway specific proteins. There are around 80 interested target proteins. For some of the proteins (Gene IDs /AGI) I have qTOF libraries (around 20). So for others I have to create theoretical digestions as I got to know. Could you please please help me to find guidelines/tutorials for this. What are the steps I should follow to create the methods for MRM and how can screen my samples after that. Before screen the sample I got to know I have to create pool and screen the targeted proteins? |
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