Spectra libraries contain the MS2 spectra data associated with a peptide identification. These libraries can be used for searching new MS2 data against previously generated identifications, or to compare the results of existing identifications.
LabKey Server generates and exports a
redundant Bibliospec file containing spectra data from the peptides/runs you select. These files are SQLite databases, and are supported by a variety of analysis tools. Redundant libraries may contain multiple spectra for a single peptide sequence.
BlibFilter can take a redundant library as input and create a non-redundant library with consensus spectra as output. Tools like
Skyline expect non-redundant libraries.
Export Spectra for Multiple Runs
To export spectra data for whole runs at a time:
- Go to the MS2 Runs web part.
- Select the desired runs from the list.
- Click MS2 Export.
- On the Export Runs page, select BiblioSpec spectra library file and click Export.
- A spectra library file is generated and downloaded to your local machine.
Export Spectra for Selected Peptides
To export spectra data from individual peptides:
- In the Peptides (or Peptides and Proteins) web part, select the peptides of interest.
- Select Export Selected > Bibliospec.
- A spectra library file is generated and downloaded to your local machine.
Export Spectra for a Filtered List of Peptides
- In the Peptides web part, filter the list of peptides to the items of interest.
- Select Export All > Bibliospec.
- LabKey Server will generate a spectra library from the entire list of peptides (respecting the current filters) and downloaded it to your local machine.
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