In this tutorial step, we import some sample Luminex® run data using the new assay design you just created. You will enter batch, run and analyte properties for a single run contained in a Excel single file. (Other topics describe how to import a multi-file run, how to import a batch of runs at once, and how to reimport a run.)

To learn more about the properties and default settings you will accept here, review the topic: Luminex Properties.

Start the Import Process

• Return to the main page of the Luminex folder (by clicking the Luminex link near the top of the page).
• In the Files web part, open the Luminex folder, and then the Runs - Assay 100 folder.
• Select the file 02-14A22-IgA-Biotin.xls by single-clicking it.
• Click Import Data.

• In the Import Data popup window, select Use Luminex Assay 100 (the design you just created).
• Click Import.

Batch Properties

First, we enter batch properties. These are properties which are set once per batch of runs, which in this first example is only a single run.

Run Properties

Next, we can enter properties specific to this run.

Analyte Properties

While any assay may have batch or run properties, some properties are particular to the specific type of assay. Analyte properties and Well Roles defined on this page are an example for Luminex assays. Learn more in this topic: Luminex Properties.

Well Roles

If a sample appears at several different dilutions, we infer you have titrated it. During the import process for the run, you can indicate whether you are using the titrated sample as a standard, quality control, or unknown. Here, we elect to use Standard1 (the standard in the imported file) as a Standard.

In this panel you will also see checkboxes for Tracked Single Point Controls. Check a box if you would like to generate a Levey-Jennings report to track the performance of a single-point control. Learn more in the Level II tutorial which includes: Step 5: Track Analyte Quality Over Time and Track Single-Point Controls in Levey-Jennings Plots.

Analyte Properties

The Analyte Properties section is used to supply properties that may be specific to each analyte in the run, or shared by all analytes in the run. For example, you could track bead or analyte lots by setting properties here.

View Results

The data has been imported, and you can view the results.

• In the Luminex Assay 100 Runs table, click 02-14A22-IgA-Biotin.xls to see the imported data for the run.
• The data grid will look something like this:

• You can see a similar page in the interactive example. (Note that there have been exclusions applied to the example following a later step in this tutorial.)

After excluding some analytes in the next step, you will reimport this run to see that when reimporting, the properties you entered are retained simplifying subsequent imports.

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Related Topics

• Luminex Properties