Could you help me understand a little bit more about how compensation is done in CPAS? Is the formula pretty much the same as what Flow Jo uses for compensation? I assume so, because compensation in CPAS looks basically identical to compensation in Flow Jo, but I was just wondering how that is calculated. Along these lines, when we edit our compensation matrix and set the positive and negative peaks based on single color controls, one must assign a value for the positive gate and another line for the negative gate. Does CPAS look at the positive line and consider everything to the right of it positive and then look at the negative line and consider everything to the left of it negative? I guess I was just wondering why those peaks have to be set twice, given that you could just cut the histogram in half (like in Flow Jo) and it will know that cells on the left are negative and cells on the right are positive. If we should be setting two separate peaks for positive and negative populations, then what happens to the cells that fall in the middle - are they just excluded? Thanks for your help!