Is this a flow run? We do have some basic background subtraction built in to flow.
From the flow overview page, click [other settings], then click [Edit ICS Metadata]. On this page, you must supply two bits of information: (1) a value that matches for both foreground and background (e.g., the experiment run), and (2) a column and value to identify which rows are background. If multiple wells match the background, the background value will be the average of the background wells.
Once you've identified the "ICS Metadata" you'll be able to add the background values for any statistic in a flow grid views using "Customize View." Unfortunately, we don't do the subtraction of the foreground from the background automatically yet. You'll need to write a custom query to do that. I can help out writing the query. |