• Sign In
  1. Flow Experiment Runs
  2. @files

FCS File 'B9 .fcs'

Flow Demo
Run Name:@files
Analysis Folder:Flow Experiment Runs
Well Name:B9 .fcs
File Date: 2008-10-10 11:56
Well Comment:
Keyword
Value
Keywords
$BEGINDATA
3584
$BEGINSTEXT
0
$BTIM
11:56:51
$BYTEORD
4,3,2,1
$CYT
LSRII
$DATATYPE
F
$DATE
10-OCT-2008
$ENDDATA
1831003
$ENDSTEXT
0
$ETIM
11:57:15
$FIL
B9 .fcs
$MODE
L
$NEXTDATA
0
$OP
Administrator
$PAR
15
$SRC
Specimen_001
$SYS
Windows XP 5.1
$TIMESTEP
41.42
$TOT
30457
APPLY COMPENSATION
TRUE
AUTOBS
TRUE
CREATOR
FlowJo
CYTNUM
1
Day
16
EXPERIMENT NAME
PBMC FlowJo
EXPORT TIME
23-OCT-2008-12:12:14
EXPORT USER NAME
Administrator
FJ_$TIMESTEP
5.149
FSC ASF
0.82
FlowJoDemoFileID
3536021137
GUID
b63a67b3-4891-4ff8-8739-83042bf757d7
LASER1ASF
1.00
LASER1DELAY
0.00
LASER1NAME
Blue
LASER2ASF
0.75
LASER2DELAY
23.80
LASER2NAME
Violet
LASER3ASF
1.30
LASER3DELAY
-27.40
LASER3NAME
UV
LASER4ASF
0.80
LASER4DELAY
59.70
LASER4NAME
Red
PLATE ID
766b4460-8810-4bdc-87fb-7369a4f01870
PLATE NAME
96 Well - U bottom
Panel
2
SPILL
8,FITC/Alexa 488-A,PE-A,PE-Cy55-A,PE-Cy7-A,CD3 CD14 Live/Dead-A,APC/Ax 647-A,APC-Ax700-A,APC-H7-A,1,0.16643300787482143,0.009606886236148684,0.0015051009909208169,0,0.00020518888830885058,0,0,0.007384218964039767,1,0.11687207968748373,0.023480652355636036,0.00008504755266136914,0.0007224623180112922,0.0006267928046597421,0.0007114916490938666,0.00025139760025828523,0.00025126988754534075,1,0.3742602095972257,0,0.049038905774422256,0.7652805220707191,0.08902127783467159,0.0008061934915472157,0.008544464271790835,0.002942178984734687,1,0,0.08605684505229083,0.10910662609106951,0.10497947698872584,0.00536910186265687,0.0017894617892055265,0,0,1,0,0,0,0.00009545641530928031,0,0.0026954618627686245,0.0006188017905481432,0,1,1.0535114953329914,0.08876670472835832,0.0002478312534206834,0.00014162863644431127,0.002880929008212223,0.00241808805664468,0,0.0025690168540350327,1,0.07873420636831327,0.0008340404224078871,0,0.00012356486776429765,0.017113326321679487,0.00039026099367006574,0.05145246481613921,0.17931761751577005,1
THRESHOLD
(FSC,20000).AND.(SSC,5000)
TUBE NAME
B9
WELL ID
B09
WINDOW EXTENSION
10.00
Parameter 1 -- FSC-A
$P1B
32
$P1E
0, 0
$P1G
1
$P1N
FSC-A
$P1R
262144
P1BS
0
P1DISPLAY
LIN
P1MS
0
Parameter 2 -- FSC-H
$P2B
32
$P2E
0, 0
$P2G
1
$P2N
FSC-H
$P2R
262144
P2BS
0
P2DISPLAY
LIN
P2MS
0
Parameter 3 -- FSC-W
$P3B
32
$P3E
0, 0
$P3G
1
$P3N
FSC-W
$P3R
262144
P3BS
-1
P3MS
0
Parameter 4 -- SSC-A
$P4B
32
$P4E
0, 0
$P4G
1
$P4N
SSC-A
$P4R
262144
P4BS
0
P4DISPLAY
LIN
P4MS
0
Parameter 5 -- SSC-H
$P5B
32
$P5E
0, 0
$P5G
1
$P5N
SSC-H
$P5R
262144
P5BS
0
P5DISPLAY
LIN
P5MS
0
Parameter 6 -- SSC-W
$P6B
32
$P6E
0, 0
$P6G
1
$P6N
SSC-W
$P6R
262144
P6BS
-1
P6MS
0
Parameter 7 -- FITC/Alexa 488-A
$P7B
32
$P7E
0, 0
$P7G
1
$P7N
FITC/Alexa 488-A
$P7R
263037
$P7S
CD56
P7BS
63
P7DISPLAY
LOG
P7MS
0
Parameter 8 -- PE-A
$P8B
32
$P8E
0, 0
$P8G
1
$P8N
PE-A
$P8R
263037
$P8S
CD11c
P8BS
330
P8DISPLAY
LOG
P8MS
0
Parameter 9 -- PE-Cy55-A
$P9B
32
$P9E
0, 0
$P9G
1
$P9N
PE-Cy55-A
$P9R
263037
$P9S
CD3
P9BS
213
P9DISPLAY
LOG
P9MS
0
Parameter 10 -- PE-Cy7-A
$P10B
32
$P10E
0, 0
$P10G
1
$P10N
PE-Cy7-A
$P10R
263037
$P10S
CD45RO
P10BS
2460
P10DISPLAY
LOG
P10MS
0
Parameter 11 -- CD3 CD14 Live/Dead-A
$P11B
32
$P11E
0, 0
$P11G
1
$P11N
CD3 CD14 Live/Dead-A
$P11R
263037
$P11S
7AAD
P11BS
46
P11DISPLAY
LOG
P11MS
0
Parameter 12 -- APC/Ax 647-A
$P12B
32
$P12E
0, 0
$P12G
1
$P12N
APC/Ax 647-A
$P12R
263037
$P12S
2H2
P12BS
2974
P12DISPLAY
LOG
P12MS
0
Parameter 13 -- APC-Ax700-A
$P13B
32
$P13E
0, 0
$P13G
1
$P13N
APC-Ax700-A
$P13R
263037
$P13S
CD4
P13BS
20767
P13DISPLAY
LOG
P13MS
0
Parameter 14 -- APC-H7-A
$P14B
32
$P14E
0, 0
$P14G
1
$P14N
APC-H7-A
$P14R
263037
$P14S
CD8
P14BS
1370
P14DISPLAY
LOG
P14MS
0
Parameter 15 -- Time
$P15B
32
$P15E
0, 0
$P15G
0.02414
$P15N
Time
$P15R
2048
P15BS
0
P15MS
0
 
 


FCS Files derived from this file and associated FCS Analyses:

Name Run Name Analysis Folder
FCS File 'B9 .fcs' Lesson_1.wsp Root
FCSAnalysis 'B9 .fcs' Lesson_1.wsp Root

More Graphs
Keywords from the FCS file
Download FCS file
Experiment Run Graph Details