ELISA Module Customization: Sample dilutions, using non-linear curve fit for calibration curves

General Server Forum (Inactive)
ELISA Module Customization: Sample dilutions, using non-linear curve fit for calibration curves Sarah Bertrand  2019-08-06 14:12
Status: Closed

We are running edition 19.10 of the LabKey Server.

We are currently trying to upload a few standard ELISA assays onto our LabKey server and have run into a few problems:

  1. There does not appear to be a way to change the calibration curve fit to anything but linear. Is there a setting I am missing or a work-around to fit other kinds of curves (i.e. 4 parameter curve fit) for concentration interpolation?
    --Is there a way to calculate EC50 in any assay modules and track across time in any other assay modules? I have attempted to modify a Luminex assay, but I am unable to load our data file

  2. We have several dilutions per sample on our ELISA plates. I tried to account for this by adding 'other' labels for the well assignments, but I do not see a way to incorporate this into the assay setup. I essentially need a column multiplying the interpolated concentration by the dilution factor. Additionally, I would like to be able to average the 4 wells (2 duplicates*2 dilutions) to get the average concentration and the error associated with the mean.
    Is there a way to post-process concentrations in the ELISA module or some kind of work around for it?

Thank you!

Jon (LabKey DevOps) responded:  2019-08-20 16:59
Hi Sarah,

The ELISA Assay Module has not really changed all that much since its inception a few years ago, so out of the box, there is no way to accommodate things such as adjusting for anything other than linear curves or do to post-process concentration adjustments. You might be able to utilize our standard GPAT assay along with using transformation scripts to further adjust your data, but that would take some work on your part to get your data into the system and present it in a manner that you want.

If you are interested in possibly working with us to expand the functionality of the ELISA module or other alternatives that we can throughly explore, I would recommend exploring the possibility of moving to a Premium Edition of LabKey vs using the Community Edition.