how to change pipeline to use tpp 5.0 | toan nguyen | 2017-12-11 11:03 |
Status: Closed | ||
/usr/local/tpp/bin/xinteract (TPP v5.0.0 Typhoon, Build 201710251406-7644 (Linux-x86_64)) PeptideProphet options [following the '-O']: i [use icat information in PeptideProphet] f [do not use icat information in PeptideProphet] g [use N-glyc motif information in PeptideProphet] H [use Phospho information in PeptideProphet] m [maldi data] I [use pI information in PeptideProphet] R [use Hydrophobicity / RT information in PeptideProphet] F [force the fitting of the mixture model, bypass automatic mixture model checks] A [use accurate mass binning in PeptideProphet] w [warning instead of exit with error if instrument types between runs is different] x [exclude all entries with asterisked score values in PeptideProphet] l [leave alone all entries with asterisked score values in PeptideProphet] n [use hardcoded default initialization parameters of the distributions] P [use Non-parametric model, can only be used with decoy option] N [do not use the NTT model] M [do not use the NMC model] k [do not use the mass model] o [optimize f-value function f(dot,delta) using PCA (applies only to SpectraST)] G [use Gamma Distribution to model the Negatives (applies only to X!Tandem data)] E [only use Expect Score as the Discriminant(applies only to X!Tandem data, helpful for data with homologous top hits e.g. phospho or glyco)] d [report decoy hits with a computed probability based on the model learned] p [run ProteinProphet afterwards] t [do not create png data plot] u [do not assemble protein groups in ProteinProphet analysis] s [do not use Occam's Razor in ProteinProphet analysis to derive the simplest protein list to explain observed peptides] |
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