The single point plots use the FI-Bkgd data that is uploaded with the run. When the control has two records (i.e. two wells, run in duplicate), the average value is calculated and reported as MFI (Mean Fluorescence Intensity).

Upload Run with Single Point Control Tracking

When you upload an assay run, the Define Well Roles panel lists the controls available for single point tracking. To briefly explore this feature, we will re-import three of the runs we used for the standard Levey-Jennings plots. Repeat these steps for any three runs:

• Go to the Assay List and select Luminex Assay 200.
• Select the row for the last run you imported, and click Re-import Run. If you did not import all 10 runs in the tutorial, it does not need to be Notebook No 10.

• Notice that as you reimport the run, the values you entered originally are provided as defaults, making it easier to change only what you intend to change.
• Leave Batch Properties unchanged and click Next.
• Leave Run Properties unchanged and click Next.
• In Define Well Roles click the box for IH5672, the only single point control available in this sample data.

• Leave Analyte Properties unchanged and click Save & Finish.
• Repeat for two more runs.

View Levey-Jennings Plots for Single Point Controls

• Go to the Assay List and select Luminex Assay 200.
• Select View QC Reports > view single point control qc report.
• Click the Graph > link next to any row.

• In this example screenshot, there are two tracked controls from Lot 815 and five from Lot 437. Your graph may vary.
• Notice the MFI (Mean Fluorescence Intensity) column in the lower right. This value is the computed average of the FI-Bkgd value for the two wells.

As with titrated standards, you can select the Graph Parameters, antigen, isotype, and conjugate. You can also define guide sets and raise qc flags for values of the single point controls which fall outside the expected range. These options are described in detail in the prior step: Use Guide Sets to Show Expected Ranges.

View Levey-Jennings Plots from QC Reports

For quicker review of the single point control Levey-Jennings plots, you can access them directly from the QC report.

• On the Assay Dashboard, click Luminex Assay 200.
• Select View QC Report > View Single Point Control QC Report.
• Click the graph icon in the L-J Plots column of any row.
• You can quickly review the plot without visiting the full page.

• The notebook number for the row you selected is shown in red along the x-axis. In this screenshot, notebook 01.