Assay Data Analysis

LabKey's assay solutions are highlighted in this data portal, including:

  • a custom assay designs to capture the experimental results and metadata
  • a data file drop where submitters can contribute experimental results
  • quality control markers to filter out invalid data
  • analyses displayed in both visual and tabular formats

A Framework for Media Comparison

This experiment provides a framework for evaluating and optimizing media recipes for T cell cultures. Different media components and ingredient ratios can be compared against one another to help support research into T cell-based therapies, especially researchers pursuing serum-free and animal-free media formulations.

Two different media for T cell cultures are compared here. Data results from other media experiments can easily be added, if desired.

Experimental Protocol

T cells were cultured over 14 days using a generic cell culture protocol.

Media recipes compared:

  • Media X - An off the shelf chemically-derived media, with no animal components. Designed for day 7 harvesting of cells.
  • Media Y - An off the shelf chemically-derived media, with no animal components. Designed for day 14 harvesting.
  • Control - no media used in culture.

Analysis Results

Media X performance peaked at day 7 (as documented by the supplier). Media Y peaks at day 14. In terms of absolute cell production, Media Y outperformed the control and Media X.

All data presented here is fictional and was created by random number generators.

 

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