TZM Nab Assay Four Parameter

LabKey Support Forum (Inactive)
TZM Nab Assay Four Parameter awilson  2011-10-21 11:10
Status: Closed
 
In the TZM Nab assay there are options for 4 Parameter and 5 Parameter log graphing functions. We do not use the same plate layout as the assay module requires. Is there a way to build this in from SQL or R scripts to generate the IC50/80 results. What is the source code for the calculations written in and is it available?
 
 
brittp responded:  2011-10-21 11:17
Hi Aaron,

The NAb assay tool allows for customization of the plate template- you can access this feature via the 'configure templates' link in the assay design editor. Let me know if you have trouble finding it. It may be that you can use the tool as-is with some tweaks to the default plate layout. Otherwise, there isn't a way to generate these exact calculations from SQL or R, though I bet there's an R package out there that will do 4PL or 5PL curve fitting if you wanted to go that route.

The source code for the curve fits is written in Java, and is part of our open source distribution- the implementation can be found in the API module, in class org.labkey.api.assay.dilution.ParameterCurveImpl.

Thanks,
Britt