|Detection and integration of large molecules||fuyanli||2018-01-08 14:02|
I am now working on large molecules such as archaeal GDGTs (m/z: 1302, 1300, 1298, 1296, 1292). I used QExactive with ESI to detect such archaeal membrane lipids which were ionized by Na, NH4 and H. I want to try to use Skyline to integrate these compounds and calculate the peak area. But I am not sure if the peak algorithm used in Skyline is suitable for these large molecules. Is there anyway to calculate the signal to noise ratio (S/N) in Skyline? What method is used in Skyline for S/N?