Import 10 runs
Here we import 10 runs of Luminex® data to the Luminex Assay 200 design. You will repeat the steps below for each of the 10 run files in the Runs folder in the Files web part.
For this set of files, the run number will be used as the Notebook No. The run number is the first two digits of the file name.
- Click on the Luminex link on the top left to navigate to the folder's portal page
- In the Files web part, double click on the Runs - Assay 200 folder to open it
- Single click on the next run file
- Click Import Data
- Select the Luminex Assay 200 assay design
- Click Import
- For Batch Properties, leave default values alone
- Click Next
- For Run Properties (illustrated below), edit the Notebook No
- Enter the first two digits of run file name (aka the run number)
- Click Next
- For Titration Well Roles, leave default values alone
- For Analyte Properties, edit the Lot Number
- Check the Same box to give the same lot number to all analytes
- Enter "Lot 437" for all analytes when importing runs #01-05
- Enter "Lot 815" for all analytes when importing runs #06-10
- Click Save And Finish
(Optional) Review how your assay design has assisted entry of run properties
This image shows the entry page for run properties to illustrate how pre-set defaults and lookups in your assay design have eased and standardize data entry. Properties configured as lookups show drop-down lists of pre-defined options. Default values that were configured as part of the assay design (such as
Isotype) have been auto-filled. The
Notebook No property was configured to remember the last entered value. In this case, it displays "10" because this image was captured after all 10 runs were imported.
(Optional) Review notes on titrations and standards
- Standard vs. quality control
- In the Define Titration Well Roles section you see during assay import, you can mark a titration as providing both a standard and a quality control. If you check both, you will see twice as many curves when you View 4pl and 5pl curve fits later. Standard titrations are used to calculate the concentrations of samples and the values displayed in Levey-Jennings plots (see Track analyte quality over time). In contrast, quality controls are used in a lab-specific manner to track the performance of the assay over time. For this tutorial, we designate our titrated standards as standards, but not as quality controls.
- Multiple standards per analyte
- If the data for your run includes multiple standard titrations (e.g., you chose to Import multi-file runs and imported multiple files of standards), you can choose which analytes to associate with which standards. Note that you must have marked each standard as a standard in the Define Titration Well Roles section before it will appear as an option. Each standard will appear over a column of checkboxes in the Analyte Properties section. You can select more than one standard for each analyte by selecting the checkboxes in more than one standard’s column. The data for this tutorial includes only one standard, so this option is not explored here.
View the imported runs and their data
View the list of all runs
After each run is imported, you will see a growing list of runs. When you are finished, the runs list will show all ten runs. It will look like this. When examining data for this assay, you can return to the runs page at any time by clicking the View Runs link.
View all results
To see results for all imported runs, click View Results on the runs page. The resulting table of data for all runs has nearly 5,000 rows, as shown here.
View one run's results
To see the results for just one run, click one of the links in the Assay ID column on the runs page. For example, if you click 01-11A12-IgA-Biotin.xls, you will see the data just for the first run, as shown here.
Next... View 4pl and 5pl curve fits
RunPropertiesEntry_Shrunk.png RunsList_Shrunk.png
