Enter Run-Specific Properties and Import Data

This page covers run-specific parameters for NAb assays. It presumes you are working through the overall steps for importing assay data covered on the Import Assay Runs page and you have already entered batch properties.

Enter Run Properties

Run parameters will be used as metadata for all data imported as part of this Run. Properties marked with a "*" are required.

It is useful to distinguish properties used for calculation (e.g., "Dilution Factor"), association (e.g., "Participant ID") and display (e.g., "Lock Y Axis") from properties used only as record-keeping (e.g., "Experiment Performer") for the run. Actively used properties have an impact on the results produced, so merit greater consideration in choice than properties that merely help you keep records.

In the list of properties below, record-keeping parameters are shown in italics, while actively used parameters are highlighted in bold. Sample values for these properties are included only to assist you in testing out import functionality, not as recommended values.

• Name. The name of this run. If you do not enter a name, the server will use the name of the imported data file for the Run's name.
• Comments
• Cutoff Percentage (1). Required. Sample value: 50. Used for calculation.
• Cutoff Percentage (2). Sample value: 80. Used for calculation if a value is given.
• Cutoff Percentage (3). Used for calculation if a value is given.
• Virus Name
• Virus ID
• Host Cell
• Study Name
• Experiment Performer
• Experiment ID
• Incubation Time
• Plate Number
• Experiment Date
• File ID
• Lock Graph Y-Axis. Fixes the Y axis from -20 to 120%. useful for generating graphs that can easily be compared side-by side. Otherwise, axes are set to fit the data, so y axes will vary between graphs.
• Curve Fit Method. Required. The assay's run report (accessed through the details link) generates all graph, IC50, IC80 and AUC information using the selected curve fit method. You can choose from the following types of curve fits:
• Four parameter
• Five parameter
• Polynomial. This algorithm allows you to quantifying a sample’s neutralization behavior based on the area under a calculated neutralization curve, commonly abbreviated as “AUC”.
• Run Data. Required. This is the data file to import. The NAb data file is a specially formatted Excel 1997-2003 file with a .xls extension.
• Same. Selecting one of these checkboxes next to a run parameter results in the use of the same run parameters for all samples in this run.
• Participant ID, Visit ID, Date, Specimen ID. These are used for mapping your run data to study specimens, participants and visits when you copy data to a study. You will see a subset of these four properties among the run properties. The subset of properties you see depends on your choice for participant/visit mapping for the batch (the radio button you selected on the previous screen of the Assay Designer).
• Sample Description
• Initial Dilution. Required. Sample value: 20.0. Used for calculation.
• Dilution Factor. Required. Sample value: 3.0. Used for calculation.
• Method. Required. Always "Dilution" or "Concentration." Sample value: Dilution. Used for calculation.

Import data

When you have finished entering property information, follow these steps:

1. Press the “Save and Import Another Run” button to continue importing runs.
2. After you finish importing runs, press "Save and Finish" to close the batch.
3. You'll now see the NAB Run Details page listing all of the specimens that belong to this sample.