Aliquots, Derivatives, and Sample Pooling

Sample Manager Help
New samples can be created from existing samples in three distinct ways:
  • Derivatives: Derive new samples from one or more existing samples, possibly with new properties or treatments.
    • Samples may also be derived from Sources in the system.
  • Pooled Samples: Group together existing samples to make a combined pool sample for actions on the aggregate.
  • Aliquots: Split a sample into identical aliquots which retain the properties of the parent sample.
    • You may also split an aliquot into a next generation of "subaliquots" by the same process.
This topic describes the mechanisms and options for creating these new samples from existing samples:

Derive Samples from Selected Parents

If you have already created the parent samples in the system and want to create new samples with specific parents (i.e. derive new samples), you can do so from the Samples grid. (Note that if you select more than 1000 rows, the option to create samples with those parents is disabled.)

  • Select the Sample Type of interest from the main menu.
  • Select the parent sample(s) using the checkbox(es).
  • Select Create > [Name of Sample Type].
  • In the popup, if you selected multiple parent samples, specify whether you want to create:
    • Derivatives: Create multiple output samples per parent.
    • Pooled Samples: Put multiple samples into pooled outputs. Covered in the next section.
  • Add the desired number of "Derivatives per parent" by entering the number and clicking Go to Sample Creation Grid.
  • You will see the Samples you had selected prepopulated as parents in the grid, with as many rows per parent as you specified.
  • Enter remaining sample information before clicking to Finish Creating the samples.

Pool Samples

To pool a set of samples into pooled outputs, such as for testing on an aggregate of many samples, follow these steps:

  • Select the Sample Type of interest from the main menu.
  • Select the 'parent' samples using the checkboxes.
    • If you only select one parent sample, the pooling option will not be shown.
  • Select Create > [Name of Sample Type].
  • In the popup, select Pooled Samples: Put multiple samples into pooled outputs.
  • Add the desired number of New samples per parent group by entering the number and clicking Go to Sample Creation Grid.
  • You will see the Samples you had selected prepopulated as parents in every row of the grid.
  • Enter remaining sample information before clicking to Finish Creating the samples.

Create Aliquots

To create aliquot portions of samples, each with copied properties from the parent sample, follow the same initial process. If you select several samples first, each one will be aliquoted individually, i.e. you will see the desired number of rows per 'parent' with each only having the single parent.

  • Select the Sample Type of interest from the main menu.
  • Select the 'parent' sample(s) using the checkbox(es).
  • Select Create > [Name of Sample Type].
  • In the popup, select Aliquots: Create aliquot copies from each parent sample.
  • Add the desired number of Aliquots per parent by entering the number and clicking Go to Sample Creation Grid.
  • You will see the Samples you had selected prepopulated as parents in every row of the grid.
  • You cannot change parent or source types when creating aliquots.
    • Each aliquot row will have the AliquotedFrom field set to the selected 'parent' (which may itself have additional parent and source information in the system.
  • Enter any remaining sample information before clicking to Finish Creating the aliquots.

When the aliquot creation is complete, as with any other you will have a banner message telling you how many were created. They are not selected by default (the original 'parent' samples remain selected) but the banner offers you quick links to add them to storage or select them in the grid. Learn more here.

Aliquot Naming

The names of the aliquots will include the name of the 'parent' sample, including that metadata directly in the name. Aliquot names are generated by appending a dash and incrementing counter to the end of the original sample name. For example, if you aliquot the sample "Tutorial-30", then "Tutorial-30-5" is the 5th aliquot. If you create a new set of aliquots later, the incrementing numbers will continue to help you clearly track the total number of aliquots of your sample.

Work with Aliquots

Show Samples, Aliquots, or Both in Grids

To make it easier to work with aliquots, all sample grids include an "Is Aliquot" column, showing 'true' for aliquots. You can use grid filtering on this column in the omnibox, or use the checkboxes on the Show Samples menu above the grid to control whether to see:

  • All Samples (includes Aliquots)
  • Samples Only
  • Aliquots Only

The name of this menu will reflect the current selections, and the filter will be shown if applicable. For example, Aliquots Only would look like:

View Aliquots of a Sample

When viewing sample details (click the name of the sample on the grid) you will see a panel showing Aliquot Details (if any).

  • Total aliquots created
  • Available vials (based on whether vials are both "In storage" and not checked out)
  • Current amount available (based on the combined stored amounts for all aliquots)

On the sample details for the originating sample, the Aliquots tab contains a grid showing all the aliquots of that sample, making it easy to track and perform storage operations on the group.

When you are viewing sample details for an aliquot it will look like any other sample, but with additional Aliquot Data on the Overview panel, including the sample it was aliquoted from. The Aliquots panel and tab will show any subaliquots of this aliquoted sample.

Aliquots in Lineage

The Lineage tab for a sample that has aliquots will show them as 'children' in the graph. Use the Filter menu to select whether to include Derivatives, Sample Parents, and Aliquots in the display.

Related Topics


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