First, we enter batch properties. These are properties which are set once per batch of runs, which in this first example is only a single run.
Next, we can enter properties specific to this run.
While any assay may have batch or run properties, some properties are particular to the specific type of assay. Analyte properties defined on this page are an example.
If a sample appears at several different dilutions, we infer you have titrated it. During the import process for the run, you can indicate whether you are using the titrated sample as a standard, quality control, or unknown.
A standard is typically the titration used in calculating estimated concentrations for unknowns based on the standard curve. A quality control is typically a titration used to track values like AUC and EC50 over time for quality purposes. Learn more in Luminex Calculations.
Here, we elect to use Standard1 (the standard in the imported file) as a Standard.
In this panel you will also see checkboxes for Tracked Single Point Controls. Check a box if you would like to generate a Levey-Jennings report to track the performance of a single-point control. Learn more in the Level II tutorial which includes: Step 5: Track Analyte Quality Over Time and Track Single-Point Controls in Levey-Jennings Plots.
The Analyte Properties section is used to supply properties that may be specific to each analyte in the run, or shared by all analytes in the run. They are not included in the data file, so need to be entered separately. For example, these properties may help you track the source of the beads used in the experiments, for the purpose of quality control. In the second tutorial we will track two lots of analytes using these properties.
The data has been imported, and you can view the results.
Note that some views of Luminex assay data will have filters already applied. These are listed in the Filter: panel above the data grid. Hover over the panel to see action buttons including Clear All if you want to remove these filters.
After excluding some analytes in the next step, you will reimport this run to see that when reimporting, the properties you entered are retained simplifying subsequent imports.