• import sequencing data from a PacBio Sequencer, including sample sheets, barcode identifiers, and table structures
• link sample sets and runs and organize with other information such as ids, types, tags, etc.
• keep different pool data (such as from different lanes) separate
• store multiple fastq files per barcode identifier, and accept fastq files without assuming the need for matching read counts
• browse and export the sequence files

Set Up a Dashboard

First we will set up a Genotyping dashboard, and import some sample data.

Next, configure the necessary queries and load reference sequences:

Load some sample data:

• Download and unzip FilesFromPacBioInstrument.zip - the PacBio sample data - to the location of your choice.
• Click Genotyping Dashboard.
• Under Tasks, click Import Run.
• Drag and drop the pacbio8 folder into the upload area (located in the FilesFromPacBioInstrument package you downloaded).
• Navigate to and select a SampleSheet.csv file. You can find one in each pool of fastq files in the sample data you just uploaded. For instance: pacbio8/pool1_barcoded_fastqs/SampleSheet.csv

• In the pop-up, scroll down to select Import PacBio Reads and click Import.
• Select the Associated Run, and optionally provide a FASTQ Prefix.
• Click Import Reads.
• Evaluate any errors received. For example, the error "Failure to send success notification, but job has completed successfully" can be disregarded.
• Click Genotyping Dashboard when the import is complete.
• Click View Runs and then the run number to see the (small) results from this sample import.

• Click a Sample ID to see the samples associated with this run.