Biologics Tutorial: Work with Mixtures and Batches

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This tutorial works with media and media recipes. The tutorial shows you how to:

  • Register a new media mixture. In the Biologics terminology, a "mixture" is a recipe, i.e., instructions for mixing together ingredients. A mixture is a virtual entity, not a physical thing that you can place in a bottle.
  • Register a physical batch of the mixture. In the Biologics terminology, a "batch" is a physical thing, i.e., the physical result of following the instructions in a recipe/mixture.

Register a Mixture

Mixtures are designed to register lab recipes and formulations. Their components are ingredients and protocol descriptions.

  • From the main menu, under Media, click Mixtures.
  • Click Add.
  • On the Details tab, enter the following:
    • Mixture Name: PBS - Cold Spring
    • Description: Cold Spring formulation with optional ingredients.
    • Alias: cold spring
    • Mixture Type: Solution
    • Click Next.
  • On the Ingredients tab, enter the following:
    • Recipe Measure Type: Volume
    • Expiration Time: 90 Days
    • Ingredients: Known
    • Click Bulk Upload.
    • In the pop-up dialog, copy-and-paste the following recipe into the text box.
TypeIngredient/MixtureAmountUnit Type
IngredientSodium Chloride80g/L
IngredientPotassium Chloride2g/L
IngredientSodium Phosphate Dibasic Anhydrous14.4g/L
IngredientPotassium Phosphate Monobasic2.4g/L
IngredientCalcium Chloride, Dihydrate, Granular1.33g/L
IngredientMagnesium chloride Anhydrous1g/L
    • Notice that you could either append these ingredients to any you'd already entered or replace any existing ingredients.
    • Click Add Ingredients to submit the recipe.
    • Click Next.
  • On the Preparation tab:
    • Click Add Step.
    • In Step Description enter the following:
      Dissolve the reagents in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.
    • You could of course split this procedure into multiple steps, but that is unnecessary for this tutorial.
    • Click Next.
  • On the Confirm tab:
    • Review the entries and click Finish.

Create a Batch

  • Select Batches from the main menu or the tabs at the top of the page.
  • Click Add.
  • On the Details tab, enter (or select) the following:
    • Mixture Used: PBS - Cold Spring
    • Batch Name: Enter "Batch for Project H", or leave this field blank and Biologics will generate a batch name for you.
    • Alias: Leave blank.
    • Description: Enter "Progress evaluation of Project H"
    • Vendor/Part No. : Leave blank.
    • Consumed: Leave unchecked.
    • Click Next.
  • On the Preparation tab, enter the following:
    • Desired Batch Yield: 1
    • Use the dropdown to select: "L"
    • Actual Batch Yield: 1
    • Expiration Date: Note that this has been auto-filled, based on the Mixture's expiration property.
    • Materials/Ingredient Source: Known
    • Amounts/Raw Materials: Known
    • Click Bulk Upload.
    • Copy-and-paste the following materials list into the text box, overwriting the pre-populated text. For simplicity, we have used 'unknown' raw ingredients instead of specific lot numbers already known to the registry.
IngredientAmount UsedRaw Material Used
Sodium Chloride80unknown
Potassium chloride2unknown
Sodium phosphate dibasic anhydrous14.4unknown
Potassium Phosphate monobasic2.4unknown
Calcium Chloride, Dihydrate, Granular1.33unknown
Magnesium chloride anhydrous1unknown
    • Click Add Ingredients to submit the list.
    • Notice that the selection for Amounts/Raw Materials changed to Unknown.
    • Click Next.
  • On the Confirm tab, click Finish.
  • A details page for the new batch is displayed.
  • Click Batches
  • A table of batches is shown, including the new batch added in this tutorial.

Tutorial Curriculum

Biologics Tutorial: Register Samples

Biologics Tutorial: Create a New Biologics Project

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