Plotting standard curves for several Luminex® runs together helps visualize any inconsistencies in data and curve fits between runs. The resulting overlay plot is sometimes called a curve "graveyard."
Here, we generate an overlay plot for the 4pl standard titration curves for the same data used in the previous steps (
Step 5: Track Analyte Quality Over Time and
Step 6: Use Guide Sets for QC).
Steps
If you navigated away after the last tutorial step, return to the Levey-Jennings plot for the ENV2 standard
- Select (Admin) > Manage Assays in the Luminex folder.
- Click Luminex Assay 200.
- Select View QC Report > view levey-jennings reports.
- Click Standard1.
- In the Choose Graph Parameters box, select Antigen "ENV2", Isotype "IgA", Conjugate "Biotin" and click Apply.
Next, create the overlay plot:
- Scroll down to the Standard1 Tracking Data for ENV2 - IgA Biotin table.
- Select all rows. (Click the box at the top of the left hand column.)
- Click View 4pl Curves to generate the overlay plot.
In the
Curve Comparison popup, you can customize what is shown:
- Y-Axis: Use the pulldown to specify what is plotted on the Y-axis. Options: FI, FI-Bkgd, FI-Bkgd-Neg
- Scale: Choose linear or log scale.
- Legend: Use the pulldown to specify how the legend is labeled.
- Export to PDF - Export the overlay plot. The exported pdf includes both linear and log versions. View a sample here.
- Close the plot when finished.
Congratulations! You have completed the Luminex Tutorial Level II.
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