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This tutorial can be completed using a free 30-day trial version of LabKey Server.

The Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method for analysis of antigen-specific responses at the cellular level and is widely used to monitor immune responses in humans and other animals. A variety of instruments provide raw ELISpot data, including CTL, Zeiss, and AID. LabKey Server provides a built in ELISpot assay type to support these commonly used assay machines. You can use the built-in assay type, as shown in this tutorial, or you can customize it to your specifications.

You can see sample ELISpot data in our interactive example.

Tutorial Steps

First Step

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