Neutralizing Antibody (NAb) assays are plate-based and can consist of either high- or low-throughput formats with dilutions either across multiple plates or within a single plate. Further, multiple viruses and associated controls may be configured on a given plate template.

This tutorial walks you through the process of creating a NAb assay design, including defining a plate template, then importing some sample data and reviewing options for working with it. Our sample data here came from a high-throughput 384 well plate with dilution across a single plate. When you input this high-throughput data, you have the option to bypass some data entry with an uploadable metadata file. If you are specifically interested in low-throughput NAb assays, you can also review the walkthrough in Work with Low-Throughput NAb Data.

Tutorial Steps

First Step


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