] [Tutorial: Import a Flow Workspace
] [Flow Demo
] [Advanced Flow Demo: Peptide Validation
The LabKey Flow module automates high-volume flow cytometry analysis. It is designed to manage large data sets from standardized assays spanning many instrument runs that share a common gating strategy.
To begin using LabKey Flow, an investigator first defines a gate
template for an entire study using FlowJo
, and uploads the FlowJo
workspace to the LabKey Server. He or she then points LabKey Flow to a
repository of FCS files on a network file server, and starts an analysis.
LabKey Flow computes the compensation matrix, applies gates, calculates
statistics, and generates graphs. Results are stored in a relational
database and displayed using secure, interactive web pages.
can then define custom queries and views to analyze large result sets.
Gate templates can be modified, and new analyses can be run and
compared. Results can be printed, emailed, or exported to tools such as
Excel or R
for further analysis. LabKey Flow enables quality control and statistical
positivity analysis over data sets that are too large to manage
effectively using PC-based solutions.
LabKey Flow is not well-suited for highly interactive, exploratory
investigations with relatively small sample sizes. We recommend FlowJo
for that type of analysis. LabKey Flow is in production use at the McElrath Lab
at FHCRC and the Wilson Lab
at the University of Washington.