During upload of Luminex files, we perform substitutions for certain flagged values. Other types of flagged values are imported without alteration.
Substitutions During Import for *[number] and OOR
We perform substitutions when Obs. Conc. is reported as OOR or *[number], where [number] is a numeric value. *[number] indicates that the measurement was barely out of range. OOR< and OOR> indicate that measurements were far out of range.
To determine the appropriate substitution, we first determine the lowest and highest "valid standards" for this analyte using the following steps:
N.B. The Conc in Range field will be *** for values flagged with * or OOR.
In the following table, the Well Dilution Factor and the Well FI refer to the Analyte Well (the particular experiment) where the Obs. Conc. was reported as OOR or as *[number].
|When Excel Obs. Conc. is...||We report Obs. Conc. as...||Where [value] is...|
|OOR <||<< [value]||the Well Dilution Factor X the Obs. Conc. of the lowest valid standard|
|OOR >||>> [value]||the Well Dilution Factor X the Obs. Conc of the highest valid standard.|
|*[number] and Well FI is less than the lowest valid standard FI||< [value]||the Well Dilution Factor X the Obs. Conc. of the lowest valid standard.|
|*[number] and Well FI is greater than the highest valid standard FI||> [value]||the Well Dilution Factor X the Obs. Conc of the highest valid standard.|
If a valid standard is not available (i.e., standard values are out of range), [value] is left blank because we do not have a reasonable guess as to the min or max value.
Flagged Values Imported Without Change
|---||Indicates that the investigator marked the well(s) as outliers||Appears in FI, FI Bkgd and/or Obs. Conc.|
|***||Indicates a Machine malfunction||Appears in FI, FI Bkgd, Std. Dev, %CV, Obs. Conc., and/or Conc.in Range|
|[blank]||No data||Appears in any column except Analyte, Type and Well, Outlier and Dilution.|
|expand all collapse all|