Several types of assays use plate-based technologies where spots or beads of sample are arrayed across a fixed size plate and read by an instrument. Creating an assay design for a plate-based technology adds the creation of a plate template to the general assay procedure outlined in Design a New Assay
or used in the Tutorial: Design a General Purpose Assay Type (GPAT)
. For a tutorial using a plate-based assay, see NAb Assay Tutorial
Recall that when you create an assay design, you name and optionally customize a specific instance of one of the built-in assay types
. There are specific types for several plate-based instruments:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Enzyme-Linked Immunosorbent Spot Assay (ELISpot)
- Neutralizing Antibody Assays (NAb)
When you create any type of plate-based assay design, the assay properties section includes a place to specify the template used to map spots or wells on the plate to data fields. The dropdown list of available templates is populated by creating named templates in the folder, project, or shared project. A variety of pre-defined templates is available; even if you make no changes to the default, you must still create a new named version.
- Click Configure Templates.
- Select one of the plate templates available.
Now you can select the template you just defined when you create an assay design.
- From the Assay List, click New Assay Design.
- Select the appropriate assay type and location.
- Click Next.
- Name the assay design (required).
- The Plate Template pulldown will include the new template as well as any others available.
- Make other assay design changes as required.
- Click Save & Close.
Edit Plate Templates
Detailed instructions on using the plate template editor can be found here:
Plate-Based Assay Examples
For a detailed walkthrough of using a plate template, try one of these tutorials: