For our sample data, we will use three mzXML files from the paper 'Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane' (Marelli et al.)
. One of the research goals was to identify proteins associated with peroxisomes that were not previously associated.
- You will need administrative permissions to complete this tutorial.
- Your server must have the MS2 module installed. To check if the MS2 module is installed, go to (Admin) > Site > Admin Console. Click Module Information. In the list of installed modules, confirm that the MS2 module is present.
- The MS2 module is currently not available with LabKey trial servers, but it is available on a virtual machine trial instance.
- If you are building the server from source code, you must build the MS2 module (which is included by default) and you must additionally obtain the proteomics analysis tools using these instructions.
Obtain the Sample MS2 Data Files
Download the sample data files. (Choose either zip or tar format, whatever is most convenient for you.)
Create a Proteomics Folder on LabKey Server
Create a new project or folder inside of LabKey server to store the demo data.
- Log in to your server and navigate to your "Tutorials" project. Create it if necessary.
- If you don't already have a server to work on where you can create projects, start here.
- If you don't know how to create projects and folders, review this topic.
- Create a new subfolder named "Proteomics Tutorial". Choose the folder type "MS2."
Upload the Sample Files
Upload the sample files you downloaded to the server's file repository following these instructions.
- Navigate to the Proteomics Tutorial folder.
- Select (Admin) > Go To Module > FileContent.
- Drag and drop the contents of the folder ProteomicsDemo into the file repository window.
- Note 1: Do not upload the parent directory, ProteomicsDemo, as the server expects these sample files to be directly in the root of the file repository.
- Note 2: There is a .labkey directory included which may be hidden on some local filesystems. Make sure you include this directory when you upload the contents. It will not show in the LabKey file browser, but must be included in what you upload. If you can't see it, try one of these:
- The sample files will be uploaded and appear in the file browser.